| 產(chǎn)品編號 | bsm-52401R |
| 英文名稱 | MKLP1 Recombinant Rabbit mAb |
| 中文名稱 | 有絲分裂驅(qū)動(dòng)蛋白樣1重組兔單抗 |
| 別 名 | CHO 1; CHO1; KIF 23; KIF23; KIF23_HUMAN; Kinesin family member 23; Kinesin like 5; Kinesin like protein 5; Kinesin like protein KIF 23; Kinesin like protein KIF23; Kinesin-like protein 5; Kinesin-like protein KIF23; KNS L5; KNSL 5; KNSL5; Mitotic kinesin like 1; Mitotic kinesin like protein 1; Mitotic kinesin-like protein 1; MKLP 1. |
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Specific References (1) | bsm-52401R has been referenced in 1 publications.
[IF=13.417] Kari L. Price. et al. Evolutionarily conserved midbody remodeling precedes ring canal formation during gametogenesis. DEV CELL. 2023 Mar 09 ICC ; Mouse.
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| 研究領(lǐng)域 | 心血管 細(xì)胞生物 細(xì)胞周期蛋白 細(xì)胞分化 細(xì)胞骨架 |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | 7C9 |
| 交叉反應(yīng) | Human,Rat (predicted: Mouse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-1000,Flow-Cyt=2ug/Test,ICC/IF=1:50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 110 kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 細(xì)胞核 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human MKLP1 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The protein encoded by this gene is a member of kinesin-like protein family. This family includes microtubule-dependent molecular motors that transport organelles within cells and move chromosomes during cell division. This protein has been shown to cross-bridge antiparallel microtubules and drive microtubule movement in vitro. Alternate splicing of this gene results in two transcript variants encoding two different isoforms. Function: Component of the centralspindlin complex that serves as a microtubule-dependent and Rho-mediated signaling required for the myosin contractile ring formation during the cell cycle cytokinesis. Essential for cytokinesis in Rho-mediated signaling. Required for the localization of ECT2 to the central spindle. Plus-end-directed motor enzyme that moves antiparallel microtubules in vitro. Subunit: Heterotetramer of two molecules each of RACGAP1 and KIF23. Found in the centralspindlin complex composed of RACGAP1 and KIF23. Interacts with RACGAP1; the interaction is direct. Interacts with ECT2 and PRC1. Interacts with ANXA11 during cytokinesis. Interacts with BIRC6/bruce and USP8/UBPY. Subcellular Location: Nucleus. Cytoplasm, cytoskeleton, spindle. Note=Localizes to the interzone of mitotic spindles. Post-translational modifications: Ubiquitinated. Deubiquitinated by USP8/UBPY. Similarity: Belongs to the kinesin-like protein family. Contains 1 kinesin-motor domain. SWISS: Q02241 Gene ID: 9493 Database links: Entrez Gene: 9493 Human Entrez Gene: 71819 Mouse Omim: 605064 Human SwissProt: Q02241 Human Unigene: 270845 Human Unigene: 259374 Mouse Unigene: 63734 Rat |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Rat Lymph node tissue lysates
Lane 2: Rat Cerebrum tissue lysates
Lane 3: Rat Testis tissue lysates
Primary: Anti-MKLP1 (bsm-52401R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size:
Sample:
Lane 1: 293 cell lysate
Lane 2: A549 cell lysate
Primary: Anti-MKLP1 (bsm-52401R) at 1:500 dilution
Secondary: Goat Anti-Rabbit IgG - HRP at 1:5000 dilution
Predicted band size: 110 kD
Observed band size: 110 kD
A549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (MKLP1) Monoclonal Antibody, Unconjugated (bsm-52401R) 1:50, 90 minutes at 37°C; followed b
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (MKLP1) Monoclonal Antibody, Unconjugated (bsm-52401R) 1:50, 90 minutes at 37°C; followed b
Blank control:A549.
Primary Antibody (green line): Rabbit Anti-MKLP1 antibody (bsm-52401R)
Dilution: 2ul/Test;
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 0.5ug/Test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)
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