| 產(chǎn)品編號 | bs-42007R |
| 英文名稱 | Rabbit Anti-NDV HN antibody |
| 中文名稱 | 雞新城疫血凝素抗體 |
| 別 名 | hemagglutinin-neuraminidase protein; newcastle disease virus(NDV); HN protein; hemagglutinin-neuraminidase protein [Newcastle disease virus]; HN_NDVB; Newcastle disease virus HN protein; Newcastle disease virus hemagglutinin neuraminidase protein. |
| 研究領域 | 免疫學 細菌及病毒 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,NDV |
| 產(chǎn)品應用 | WB=1:500-2000,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 63 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Recombinant NDV HN protein: 49-577/577 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The entry of Newcastle disease virus (NDV), a prototype paramyxovirus, is directed by two virion glycoproteins, the hemagglutinin-neuraminidase (HN) protein and the fusion (F) protein . HN protein, the virus attachment protein, binds to sialic acid-containing receptors, and F protein mediates membrane fusion. In contrast to many viral fusion proteins, paramyxovirus F proteins do not require the acid pH of endosomes to activate fusion activity. As a consequence, infected cells expressing both attachment proteins and F proteins can fuse with adjacent cells to form multinuclear cells, or syncytia, a process that is assumed to mimic virus-cell fusion . Database links: SwissProt: P32884 NDV |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Recombinant NDV HN protein, His
Primary: Anti-NDV HN (bs-42007R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 63 kDa
Observed band size: 61 kDa
Measured by its binding ability in a indirect ELISA. Immobilized Human NDV HN protein, His Tag (Cat. bs-42007P) at 2 μg/mL (100 μL/well) can bind ?Rabbit Anti-Human NDV HN Antibody, the EC50 is 51.59 ng/mL.
Measured by its binding ability in a indirect ELISA. Immobilized Human NDV HN protein, His Tag (Cat. bs-42007P) at 2 μg/mL (100 μL/well) can bind ?Rabbit Anti-Human NDV HN Antibody, the EC50 is 54.90 ng/mL.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
