| 產(chǎn)品編號(hào) | bsm-60400M |
| 英文名稱 | NRF1 Recombinant Mouse mAb |
| 中文名稱 | 核呼吸因子-1重組鼠單抗 |
| 別 名 | erythroid derived 2; like 1; Locus control region factor 1; Locus control region-factor 1; NF-E2-related factor 1; NF2L1_HUMAN; NFE2 related factor 1; NFE2-related factor 1; NFE2L1; NRF-1; NRF 1; Nuclear factor; Nuclear factor erythroid 2-related factor 1 |
| 研究領(lǐng)域 | 染色質(zhì)和核信號(hào) 生長因子和激素 轉(zhuǎn)錄調(diào)節(jié)因子 |
| 抗體來源 | Mouse |
| 克隆類型 | Recombinant |
| 交叉反應(yīng) | Human |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,ICC/IF=1:100
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 55 kDa |
| 檢測(cè)分子量 | |
| 細(xì)胞定位 | 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human NRF1 |
| 亞 型 | IgG2b/Kappa |
| 純化方法 | affinity purified by Protein G |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes a protein that homodimerizes and functions as a transcription factor which activates the expression of some key metabolic genes regulating cellular growth and nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. The protein has also been associated with the regulation of neurite outgrowth. Alternative splicing results in multiple transcript variants. Confusion has occurred in bibliographic databases due to the shared symbol of NRF1 for this gene and for "nuclear factor (erythroid-derived 2)-like 1" which has an official symbol of NFE2L1. [provided by RefSeq, May 2014] SWISS: Q16656 Gene ID: 4899 |
| 產(chǎn)品圖片 |
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 4°C overnight
Secondary Ab: Goat Anti-Mouse IgG H&L (HRP)
Lysate: 1: HeLa, 2: HEK-293, 3: Jurkat
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 54 kDa
Observed MW: 70 kDa
Sample:
Lane 1: Human Hela cell lysates
Lane 2: Human HepG2 cell lysates
Lane 3: Human SH-SY5Y cell lysates
Lane 4: Human 293T cell lysates
Lane 5: Human Jurkat cell lysates
Primary: Anti- NRF1 (bsm-60400M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 55 kDa
Observed band size: 70 kDa
Cell line: HepG2
Fixative: 4% Paraformaldehyde
Permeabilization: 0.1% TritonX-100
Primary Ab dilution: 1:50
Primary incubation condition: 4°C overnight
Secondary Ab: Goat Anti-Mouse IgG
Nuclear counter stain: DAPI (Blue)
Counter stain: Tubulin (Red)
Comment: Color green is the positive signal for bsm-60400M
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