| 產(chǎn)品編號(hào) | bsm-51666M |
| 英文名稱(chēng) | GNMT Mouse mAb |
| 中文名稱(chēng) | 甘氨酸-N-甲基轉(zhuǎn)移酶單克隆抗體 |
| 別 名 | EC 2.1.1.20; Epididymis secretory sperm binding protein Li 182mP; Glycine N methyltransferase; Glycine N-methyltransferase; GNMT_HUMAN; HEL S 182mP; OTTHUMP00000016412. |
| 研究領(lǐng)域 | 信號(hào)轉(zhuǎn)導(dǎo) 新陳代謝 |
| 抗體來(lái)源 | Mouse |
| 克隆類(lèi)型 | Monoclonal |
| 克 隆 號(hào) | K8T2 |
| 交叉反應(yīng) | Human |
| 產(chǎn)品應(yīng)用 | WB=1:500-4000,Flow-Cyt=1:25
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 32 kDa |
| 檢測(cè)分子量 | |
| 細(xì)胞定位 | 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Recombinant human GNMT between 1-295 amino acids. |
| 亞 型 | IgG2a,k |
| 純化方法 | affinity purified by Protein G |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
GNMT catalyzes the methylation of glycine by using S-adenosylmethionine (AdoMet) to form N-methylglycine (sarcosine) with the concomitant production of S-adenosylhomocysteine (AdoHcy). It plays a possible crucial role in the regulation of tissue concentration of AdoMet and of metabolism of methionine. Function: Catalyzes the methylation of glycine by using S-adenosylmethionine (AdoMet) to form N-methylglycine (sarcosine) with the concomitant production of S-adenosylhomocysteine (AdoHcy). Possible crucial role in the regulation of tissue concentration of AdoMet and of metabolism of methionine. Subunit: Homotetramer. Subcellular Location: Cytoplasm. Tissue Specificity: Abundant in liver. DISEASE: Defects in GNMT are the cause of glycine N-methyltransferase deficiency (GNMT deficiency) [MIM:606664]; also known as hypermethioninemia. The only clinical abnormalities in patients with this deficiency are mild hepatomegaly and chronic elevation of serum transaminases. Similarity: Belongs to the pyridoxine kinase family. SWISS: Q14749 Gene ID: 27232 Database links: Entrez Gene: 27232 Human SwissProt: Q14749 Human |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Human liver tissue lysates
Primary: Anti-GNMT (bsm-51666M) at 1/4000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 32 kD
Observed band size: 34 kD
Blank control:U-2 OS.
Primary Antibody (green line): Mouse Anti-GNMT antibody (bsm-51666M)
Dilution: 1:25;
Isotype Control Antibody (blue line): Mouse IgG
Secondary Antibody : Goat anti-mouse IgG-AF488
Dilution: 1:400
Protocol
The cells were fixed wi
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