| 產(chǎn)品編號 | bs-21165R |
| 英文名稱 | RASSF10 Rabbit pAb |
| 中文名稱 | RASSF10蛋白抗體 |
| 別 名 | 4632411J06Rik; Ras association(RalGDS/AF 6) domain family(N terminal) member 10; Ras association(RalGDS/AF-6) domain family(N-terminal) member 10; ras association domain containing protein 10; Ras association domain-containing protein 10; RASFA_HUMAN; RAS |
| 研究領(lǐng)域 | 細胞生物 信號轉(zhuǎn)導 G蛋白信號 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Mouse (predicted: Human,Rat,Rabbit,Pig,Cow,Chicken,Dog) |
| 產(chǎn)品應用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 57 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human RASSF10: 51-150/507 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | Preservative: 0.02% Proclin300, Constituents: 1% BSA, 0.01M PBS, pH7.4. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
RASSF10 (Ras Association Domain Family Member 10) is a Protein Coding gene. An important paralog of this gene is RASSF9. Tissue Specificity: Expressed in brain. Tends to be down-regulated in astrocytic gliomas due to promoter methylation. Methylation occurs early in gliomagenesis and the extent of methylation parallels with higher glioma grades, so that methylation is observed in close to 70% WHO grade IV primary glioblastomas, but not in grade I astrocytomas. DISEASE: During interphase, predominantly cytoplasmic, although some nuclear staining in several tumor cell contexts. During prophase, observed at developing centrosomes. Displays persistent localization with centrosomally radiating microtubule bundles until late telophase. Associates with spindle poles particularly during metaphase and anaphase before relocating back to the cytoplasm. Similarity: Contains 1 Ras-associating domain. SWISS: A6NK89 Gene ID: 644943 Database links: Entrez Gene: 644943 Human SwissProt: A6NK89 Human Unigene: 134650 Human |
| 產(chǎn)品圖片 |
Sample:
Testis (Mouse) Lysate at 40 ug
Primary: Anti-RASSF10 (bs-21165R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 57 kD
Observed band size: 57 kD
Sample:
Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti-RASSF10 (bs-21165R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 57 kD
Observed band size: 57 kD
Sample:
Lung (Mouse) Lysate at 40 ug
Primary: Anti-RASSF10 (bs-21165R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 57 kD
Observed band size: 57 kD
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
