| 產(chǎn)品編號 | bs-20047R |
| 英文名稱 | ELMO2 Rabbit pAb |
| 中文名稱 | 吞噬細(xì)胞運動蛋白2抗體 |
| 別 名 | CED 12; Ced 12 homolog 2; CED 12 homolog A; CED 12A; CED-12; ced-12 homolog 2; CED12; CED12 homolog A; CED12A; ELMO 2; ELMO-2; ELMO2; ELMO2_HUMAN; Engulfment and cell motility 2(ced 12 homolog, C. elegans); Engulfment and cell motility 2; Engulfment and c |
| 研究領(lǐng)域 | 細(xì)胞生物 信號轉(zhuǎn)導(dǎo) 細(xì)胞凋亡 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human (predicted: Mouse,Rat,Rabbit,Pig,Cow,Horse) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 83 kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 細(xì)胞漿 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ELMO2: 101-200/720 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Elmo (engulfment and cell motility) proteins share similarity to C. elegans CED-12. The C. elegans genes ced-2, ced-5, ced-10, and ced-12 and their mammalian homologs CRKII, DOCK1, RAC1 and ELMO mediate cytoskeletal rearrangements during phagocytosis of apoptotic cells and cell motility. Elmo1 associates with DOCK 180 and may influence phagocytosis and effect cell shape changes. Src family kinase mediated tyrosine phosphorylation of ELMO1 influences signaling through Elmo1/Crk/DOCK 180 pathways. Elmo2 interacts directly with Rho G in a GTP-dependent manner and forms a ternary complex with DOCK 180 to induce activation of Rac 1. The RhoG-Elmo2-DOCK 180 pathway is required for activation of Rac 1 and cell spreading mediated by integrin, as well as for neurite outgrowth induced by nerve growth factor. Elmo3 acts in assocation with DOCK180 and Crk II and may be required in complex with DOCK180 to activate Rac/Rho small GTPases. Function: Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Acts in assocation with DOCK1 and CRK. Was initially proposed to be required in complex with DOCK1 to activate Rac Rho small GTPases. May enhance the guanine nucleotide exchange factor (GEF) activity of DOCK1. Subunit: Interacts with the SH3-domain of DOCK1 via its SH3-binding site. Probably part of a complex with DOCK1 and RAC1. Probably part of a complex with DOCK1 and CRK isoform CRK-II. Interacts with ARHGEF16, DOCK4 and EPHA2; mediates activation of RAC1 by EPHA2. Subcellular Location: Cytoplasm. Cytoplasm; cytosol. Membrane. Tissue Specificity: Widely expressed, with a higher expression in skeletal muscle, kidney and placenta. Similarity: Contains 1 ELMO domain. Contains 1 PH domain. SWISS: Q96JJ3 Gene ID: 63916 Database links: Entrez Gene: 63916 Human Entrez Gene: 140579 Mouse Omim: 606421 Human SwissProt: Q96JJ3 Human SwissProt: Q8BHL5 Mouse Unigene: 210469 Human Unigene: 35064 Mouse Unigene: 2097 Rat |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (Human pancreatic cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ELMO2) Polyclonal Antibody, Unconjugated (bs-20047R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
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