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GPX2 Rabbit pAb (bs-13396R)  
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產(chǎn)品編號(hào) bs-13396R
英文名稱 GPX2 Rabbit pAb
中文名稱 谷胱甘肽過(guò)氧化酶2抗體
別    名 Gastrointestinal glutathione peroxidase; GI GPx; Glutathione peroxidase 2(gastrointestinal); Glutathione peroxidase 2; Glutathione peroxidase gastrointestinal; Glutathione peroxidase related protein 2; Glutathione peroxidase-gastrointestinal; Glutathione   
研究領(lǐng)域 心血管  細(xì)胞生物  免疫學(xué)  神經(jīng)生物學(xué)  新陳代謝  
抗體來(lái)源 Rabbit
克隆類型 Polyclonal
交叉反應(yīng) Mouse,Rat (predicted: Human,Rabbit,Sheep,Cow,Dog,Horse)
產(chǎn)品應(yīng)用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 22 kDa
檢測(cè)分子量
細(xì)胞定位 細(xì)胞漿 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human GPX2/Glutathione Peroxidase 2: 41-140/190 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 Glutathione peroxidase (GPx) enzymes are generally selenium-containing tetrameric glycoproteins that help prevent lipid peroxidation of cell membranes. GPx enzymes reduce lipid hydroperoxides to alcohols, and reduce free hydrogen peroxide to water. GPx members are among the few proteins known in higher vertebrates to contain selenocysteine, which occurs at the active site of glutathione peroxidase and is coded by the nonsense (stop) codon TGA. There are eight GPx homologs (GPx-1–8). GPx-1 plays an important role in the antioxidant defense of the vascular wall and neural cells in response to oxidative stress. GPx-2 is the major isoform in the lungs and its basal or inducible expression is dependent on Nrf2. GPx-3 is under regulation by hypoxic stress and the expression and deficiency of GPx-3 is associated with cardiovascular disease and stroke. GPx-5 is selenium-independent; it is bound to the acrosome of sperm, where it may protect sperm from premature acrosome reaction in the epididymis.

Function:
Could play a major role in protecting mammals from the toxicity of ingested organic hydroperoxides. Tert-butyl hydroperoxide, cumene hydroperoxide and linoleic acid hydroperoxide but not phosphatidycholine hydroperoxide, can act as acceptors.

Subunit:
Homotetramer.

Subcellular Location:
Cytoplasm. Mainly cytoplasmic.

Tissue Specificity:
Mostly in liver and gastrointestinal tract, not found in heart or kidney.

Similarity:
Belongs to the glutathione peroxidase family.

SWISS:
P18283

Gene ID:
2877

Database links:

Entrez Gene: 2877 Human

Entrez Gene: 14776 Mouse

Entrez Gene: 29326 Rat

Omim: 138319 Human

SwissProt: P18283 Human

SwissProt: Q9JHC0 Mouse

SwissProt: P83645 Rat

Unigene: 2704 Human

Unigene: 441856 Mouse

Unigene: 3503 Rat



產(chǎn)品圖片
Sample: Lane 1: Mouse Liver tissue lysates Lane 2: Rat Kidney tissue lysates Primary: Anti-GPX2 (bs-13396R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 22 kDa Observed band size: 22 kDa
Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (GPX2) Polyclonal Antibody, Unconjugated (bs-13396R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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