| 產(chǎn)品編號 | bs-9898R |
| 英文名稱 | SISP1 Rabbit pAb |
| 中文名稱 | 應激誘導分泌蛋白1抗體 |
| 別 名 | C10orf54; VISTA_HUMAN; Platelet receptor Gi24; stress induced secreted protein 1; UNQ730/PRO1412; V-type immunoglobulin domain-containing suppressor of T-cell activation; Stress-induced secreted protein-1; Sisp-1; V-set domain-containing immunoregulatory |
| 研究領域 | 心血管 細胞生物 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human |
| 產(chǎn)品應用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 31 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human SISP1/GI24: 21-120/311 <Extracellular> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Platelet receptor GI24, also known as SISP1 or PP2135, is a 311 amino acid single-pass type I membrane protein that contains one Ig-like (immunoglobulin-like) domain. GI24 is thought to contribute to tumor-invasive growth in the collagen matrix and is encoded by a gene that maps to human chromosome 10q22.1. Chromosome 10 contains over 800 genes, 135 million nucleotides and comprises nearly 4.5% of the human genome. PTEN is an important tumor suppressor gene located on chromosome 10 and, when defective, causes a genetic predisposition to cancer development known as Cowden syndrome. The chromosome 10 encoded gene ERCC6 is important for DNA repair and is linked to Cockayne syndrome which is characterized by extreme photosensitivity and premature aging. Tetrahydrobiopterin deficiency and a number of syndromes involving defective skull and facial bone fusion are also linked to chromosome 10. As with most trisomies, trisomy 10 is rare and is deleterious. Subcellular Location: Membrane. Similarity: Contains 1 Ig-like (immunoglobulin-like) domain. SWISS: Q9H7M9 Gene ID: 64115 Database links: Entrez Gene: 64115 Human Entrez Gene: 74048 Mouse SwissProt: Q9H7M9 Human SwissProt: Q9D659 Mouse Unigene: 47382 Human Unigene: 273584 Mouse |
| 產(chǎn)品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with SISP1 polyclonal antibody, unconjugated (bs-9898R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
