| 產(chǎn)品編號 | bs-7109R |
| 英文名稱 | PARP4 Rabbit pAb |
| 中文名稱 | 蛋白質(zhì)單ADP核糖基轉(zhuǎn)移酶PARP4抗體 |
| 別 名 | PARP related/IalphaI related H5/proline rich; Vault poly ADP ribose polymerase; VPARP; 193kDa vault protein; ADP ribosyltransferase(NAD+poly(ADP ribose) polymerase) like 1; ADP ribosyltransferase like 1; ADPRT L1; ADPRTL 1; ADPRTL1; H5 proline rich; I alp |
 |
Specific References (2) | bs-7109R has been referenced in 2 publications.
[IF=8.44] Lu Bingfeng. et al. C/D box small nucleolar RNA SNORD104 promotes endometrial cancer by regulating the 2?-O-methylation of PARP1. J TRANSL MED. 2022 Dec;20(1):1-14 WB ; Human.
[IF=1.12] Liu et al. Keeping the heart empty and beating: an alternative technique to preserve hypertrophied hearts during valvular surgery. (2015) J.Cardiothorac.Surg. 10:71 WB ; Porcine.
|
| 研究領域 | 細胞生物 細胞凋亡 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Rat (predicted: Human,Mouse,Rabbit,Pig,Sheep) |
| 產(chǎn)品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 192 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PARP4: 851-950/1724 |
| 亞 型 | |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Component of the vault ribonucleoprotein particle which is at least composed of MVP, PARP4 and one or more vault RNAs (vRNAs). Binds to MVP. Associates with TEP1. Widely expressed; the highest levels are in the kidney; also detected in heart, placenta, lung, liver, skeletal muscle, spleen, leukocytes and pancreas. Subunit: Component of the vault ribonucleoprotein particle, at least composed of MVP, PARP4 and one or more vault RNAs (vRNAs). Binds to MVP. Associates with TEP1. Subcellular Location: Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, spindle. Note=Also found in the nucleus, associated with mitotic spindles. Tissue Specificity: Widely expressed; the highest levels are in the kidney; also detected in heart, placenta, lung, liver, skeletal muscle, spleen, leukocytes and pancreas. Similarity: Contains 1 BRCT domain. Contains 1 PARP alpha-helical domain. Contains 1 PARP catalytic domain. Contains 1 VIT domain. [SIMILARITY] Contains 1 VWFA domain. SWISS: Q9UKK3 Gene ID: 143 Database links: Entrez Gene: 143 Human Omim: 607519 Human SwissProt: Q9UKK3 Human Unigene: 117825 Human |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PARP4) Polyclonal Antibody, Unconjugated (bs-7109R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
|
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
