產(chǎn)品編號(hào) | bs-7637R |
英文名稱 | ICAM4 Rabbit pAb |
中文名稱 | 細(xì)胞間粘附分子4抗體 |
別 名 | CD242; CD242 antigen; ICAM-4; Icam4; ICAM4_HUMAN; Intercellular adhesion molecule 4; Landsteiner Wiener blood group glycoprotein; Landsteiner-Wiener blood group glycoprotein; LW; LW blood group protein. |
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Specific References (1) | bs-7637R has been referenced in 1 publications.
[IF=3.23] Polin, Helene, et al. "Compound heterozygosity of two novel RHAG alleles leads to a considerable disruption of the Rh complex." Transfusion 56.4 (2016): 950-955. FCM ; Human.
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研究領(lǐng)域 | 心血管 細(xì)胞生物 免疫學(xué) 信號(hào)轉(zhuǎn)導(dǎo) 細(xì)胞凋亡 細(xì)胞粘附分子 細(xì)胞骨架 |
抗體來(lái)源 | Rabbit |
克隆類型 | Polyclonal |
交叉反應(yīng) | Human,Mouse (predicted: Rat,Pig,Cow,Dog,Horse) |
產(chǎn)品應(yīng)用 | WB=1:500-2000,Flow-Cyt=1ug/test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理論分子量 | 27 kDa |
檢測(cè)分子量 | |
細(xì)胞定位 | 細(xì)胞膜 分泌型蛋白 |
性 狀 | Liquid |
濃 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human ICAM4: 85-180/271 <Extracellular> |
亞 型 | |
純化方法 | affinity purified by Protein A |
緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
產(chǎn)品介紹 |
ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). ICAM4 is also a ligand for alpha-4/beta-1 and alpha-V integrins. Function: ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). ICAM4 is also a ligand for alpha-4/beta-1 and alpha-V integrins. Subcellular Location: Isoform Long: Cell membrane; Single-pass type I membrane protein. Isoform Short: Secreted. Tissue Specificity: Erythrocytes. Post-translational modifications: N- and O-glycosylated. Similarity: Belongs to the immunoglobulin superfamily. ICAM family. Contains 2 Ig-like C2-type (immunoglobulin-like) domains. SWISS: Q14773 Gene ID: 3386 Database links: Entrez Gene: 3386 Human Entrez Gene: 78369 Mouse Omim: 614088 Human SwissProt: Q14773 Human SwissProt: Q9ERM2 Mouse Unigene: 706750 Human Unigene: 30220 Mouse Unigene: 222410 Rat |
產(chǎn)品圖片 |
Sample: placenta(Mouse) Lysate at 40 ug
Primary: Anti-ICAM4 (bs-7637R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 27 kD
Observed band size: 45 kD
Sample: Bone (Mouse) Lysate at 40 ug
Primary: Anti-ICAM4 (bs-7637R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 27 kD
Observed band size: 45 kD
Sample: K562 Cell (Human) Lysate at 40 ug
Primary: Anti-ICAM4 (bs-7637R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 27 kD
Observed band size: 45 kD
Blank control:K562.
Primary Antibody (green line): Rabbit Anti-ICAM4 antibody (bs-7637R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The
Blank control:K562. Primary Antibody (green line): Rabbit Anti-ICAM4 antibody (bs-7637R) Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protoco
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