| 產(chǎn)品編號 | bs-3516R |
| 英文名稱 | WBSCR14 Rabbit pAb |
| 中文名稱 | 糖類應(yīng)答元件結(jié)合蛋白ChREBP抗體 |
| 別 名 | ChREBP; bHLHd14; Carbohydrate responsive element binding protein; MIO; MLX interacting protein like; Mlx interactor; MONDOB; WBSCR 14; WBSCR14; MLXIPL; Williams Beuren syndrome chromosome region 14; Williams Beuren syndrome chromosome region 14 protein; W |
| 研究領(lǐng)域 | 腫瘤 心血管 免疫學(xué) 信號轉(zhuǎn)導(dǎo) 轉(zhuǎn)錄調(diào)節(jié)因子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Rat (predicted: Human,Mouse,Pig,Cow,Chicken,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 93 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human WBSCR14/ChREBP: 81-180/852 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
ChREBP (Carbohydrate responsive element binding protein) is a transcription factor playing a critical role in the nutrient and hormonal regulation of genes encoding enzymes of glucose metabolism and lipogenesis pathways. It contains several domains including a nuclear localization signal (NLS) near the N-terminus, polyproline domains, a basic helix-loop-helix leucine zipper (b/HLH/Zip) and a leucine zipper like (zip-like) domain.
ChREBP is ubiquitously detected in various tissues, with highest expression in liver, kidney and white and brown adipose tissue. Under basal conditions ChREBP is localized in the cytosol, translocating into the nucleus upon high glucose stimulation following its dephosphorylation of phospho-serine 196. Function: Transcriptional repressor. Binds to the canonical and non-canonical E box sequences 5'-CACGTG-3' (By similarity). Subunit: Binds DNA as a heterodimer with TCFL4/MLX. Subcellular Location: Nucleus. Tissue Specificity: Expressed in liver, heart, kidney, cerebellum and intestinal tissues. Post-translational modifications: Phosphorylation at Ser-556 by AMPK inactivates the DNA-binding activity (By similarity). DISEASE: Note=WBSCR14 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Haploinsufficiency of WBSCR14 may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in the disease. Similarity: Contains 1 basic helix-loop-helix (bHLH) domain. SWISS: Q9NP71 Gene ID: 51085 Database links: Entrez Gene: 51085 Human Omim: 605678 Human SwissProt: Q9NP71 Human Unigene: 647055 Human ChREBP（又稱碳水化合物反應(yīng)元件結(jié)合蛋白）是葡萄糖信號途徑中的一個新的轉(zhuǎn)錄因子,在哺乳動物體內(nèi)可結(jié)合到糖酵解和脂肪合成酶相關(guān)基因啟動子區(qū)的糖類應(yīng)答元件(ChRE)上, 在他們的共同作用下，可調(diào)節(jié)糖酵解和脂肪合成酶相關(guān)基因的表達. |
| 產(chǎn)品圖片 |
Tissue/cell: rat rectum tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-WBSCR14 Polyclonal Antibody, Unconjugated(bs-3516R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
